Amasai (a-măs'a-î or ăm'a-sâ-i), burdensame. 1. A Levite, one, of the sons of Elkanah. 1 Chronicles 6:25. 2. A chief of the captains who joined David in the desert. 1 Chronicles 12:18. 3. One of the Levites. 1 Chronicles 15:24. 4. The father of Mahath. 2 Chronicles 29:12.