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Letter (KJV = jot) (2503) (iota) is the ninth letter of the Greek alphabet and is used as an equivalent. Iota is the smallest letter of the Greek alphabet and is used to express smallness or the minutest part of something. Here iota is used for the jot or yod (yodh) which is the tenth and smallest letter of the Hebrew alphabet and which looks like an apostrophe ('). There are approximately 66,420 yods in the Old Testament! Jesus believed in the literal inspiration of the Bible, even in what might seem small unimportant details. Nothing in Scripture, even the smallest stroke, is without significance. Not only the words, but even the letters are divinely inspired. Vincent - Jot is for jod (or yod), the smallest letter in the Hebrew alphabet. Tittle is the little bend or point which serves to distinguish certain Hebrew letters of similar appearance. Jewish tradition mentions the letter jod as being irremovable; adding that, if all men in the world were gathered to abolish the least letter in the law, they would not succeed. The guilt of changing those little hooks which distinguish between certain Hebrew letters is declared to be so great that, if such a thing were done, the world would be destroyed. (Matthew 5 Greek Word Studies)

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